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circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k)  (Arraystar inc)

 
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    Arraystar inc circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k)
    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show <t>circRNA</t> expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.
    Circrna Expression Microarray Slide Arraystar Mouse Circrna Array V2 (8 × 15k), supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arraystar+mouse+circrna+array+v2/circrna+microarray+arraystar+human+circrna+array+v2/pmc07015875-65-15-19
    Average 90 stars, based on 1 article reviews
    circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke"

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2020.00081

    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.
    Figure Legend Snippet: Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Techniques Used: Expressing, Control, Quantitative Proteomics

    RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).
    Figure Legend Snippet: RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Techniques Used: Quantitative RT-PCR, Microarray, Quantitative Proteomics

    circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.
    Figure Legend Snippet: circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Techniques Used:

    Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).
    Figure Legend Snippet: Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Techniques Used:

    KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).
    Figure Legend Snippet: KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Techniques Used:

    Related Articles

    Control:

    Article Title: circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis.
    Article Snippet: After adding DCFH-DA diluent (1: 1000) and incubating for 20 min, the cells were washed for 3 times by PBS, which were then observed and photographed by the inverted fluorescence microscope (Olympus, Tokyo, Japan). .. AC16 cells in control group and H/RI group were collected for Arraystar Human circRNA Array V2 analysis, and mouse primary cardiomyocyte in control group and H/RI group were collected for mouse circRNA Array analysis. ..

    Article Title: circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis
    Article Snippet: .. Establishment of hypoxia-reoxygenation injury model in AC16 cells for targeted circRNAs selecting. ( A ) Cell viability of AC16 cells under different re-oxygenation times (n = 6). ( B ) LDH release of AC16 cells under different re-oxygenation times (n = 6). ( C ) Effects of H 24 h/R 1 h injury (H/RI) on cell morphology of AC16 cells (n = 3). ( D ) Effects of H/RI on ROS level in AC16 cells (n = 3). ( E ) Effects of H/RI on mRNA levels of IL-1β , IL-6 , TNF-α and ICAM-1 in AC16 cells (n = 6). ( F ) Heatmap of H/RI group compared with control group (n = 3). ( G ) The volcanic map of differentially expressed circRNAs and the candidate target circRNAs (n = 3). ( H ) Raw intensity of the candidate circRNAs in Arraystar Human CircRNA Array V2 analysis (n = 3). ( I ) RT-PCR validation results of the candidate circRNAs (n = 3). ( J ) Information of hsa_circ_0006174 (hsa_circRNA_104852) and mmu_circ_0011688. ( K ) The results of situ hybridization experiments of hsa_circ_104852 in AC16 cells and mmu_circ_ 0011688 in mouse heart tissue (n = 3). ( L ) The expression level of circMIRIAF in mouse heart tissue and mouse primary cardiomyocytes (n = 6). ..

    Microarray:

    Article Title: Circular RNA Profile in Atherosclerotic Disease: Regulation during ST-Elevated Myocardial Infarction
    Article Snippet: RNA concentrations and purity based on the 260/280 and the 260/230 ratios were assessed by spectrophotometer absorbance (NanoDrop ND-1000, Thermo Fisher Scientific, Waltham, MA, USA). .. CircRNA microarray was performed using Arraystar Mouse circRNA Array V2 (Arraystar Inc., Rockville, MD, USA) based on Arraystar’s standard protocols. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis
    Article Snippet: .. Establishment of hypoxia-reoxygenation injury model in AC16 cells for targeted circRNAs selecting. ( A ) Cell viability of AC16 cells under different re-oxygenation times (n = 6). ( B ) LDH release of AC16 cells under different re-oxygenation times (n = 6). ( C ) Effects of H 24 h/R 1 h injury (H/RI) on cell morphology of AC16 cells (n = 3). ( D ) Effects of H/RI on ROS level in AC16 cells (n = 3). ( E ) Effects of H/RI on mRNA levels of IL-1β , IL-6 , TNF-α and ICAM-1 in AC16 cells (n = 6). ( F ) Heatmap of H/RI group compared with control group (n = 3). ( G ) The volcanic map of differentially expressed circRNAs and the candidate target circRNAs (n = 3). ( H ) Raw intensity of the candidate circRNAs in Arraystar Human CircRNA Array V2 analysis (n = 3). ( I ) RT-PCR validation results of the candidate circRNAs (n = 3). ( J ) Information of hsa_circ_0006174 (hsa_circRNA_104852) and mmu_circ_0011688. ( K ) The results of situ hybridization experiments of hsa_circ_104852 in AC16 cells and mmu_circ_ 0011688 in mouse heart tissue (n = 3). ( L ) The expression level of circMIRIAF in mouse heart tissue and mouse primary cardiomyocytes (n = 6). ..

    Biomarker Discovery:

    Article Title: circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis
    Article Snippet: .. Establishment of hypoxia-reoxygenation injury model in AC16 cells for targeted circRNAs selecting. ( A ) Cell viability of AC16 cells under different re-oxygenation times (n = 6). ( B ) LDH release of AC16 cells under different re-oxygenation times (n = 6). ( C ) Effects of H 24 h/R 1 h injury (H/RI) on cell morphology of AC16 cells (n = 3). ( D ) Effects of H/RI on ROS level in AC16 cells (n = 3). ( E ) Effects of H/RI on mRNA levels of IL-1β , IL-6 , TNF-α and ICAM-1 in AC16 cells (n = 6). ( F ) Heatmap of H/RI group compared with control group (n = 3). ( G ) The volcanic map of differentially expressed circRNAs and the candidate target circRNAs (n = 3). ( H ) Raw intensity of the candidate circRNAs in Arraystar Human CircRNA Array V2 analysis (n = 3). ( I ) RT-PCR validation results of the candidate circRNAs (n = 3). ( J ) Information of hsa_circ_0006174 (hsa_circRNA_104852) and mmu_circ_0011688. ( K ) The results of situ hybridization experiments of hsa_circ_104852 in AC16 cells and mmu_circ_ 0011688 in mouse heart tissue (n = 3). ( L ) The expression level of circMIRIAF in mouse heart tissue and mouse primary cardiomyocytes (n = 6). ..

    Hybridization:

    Article Title: circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis
    Article Snippet: .. Establishment of hypoxia-reoxygenation injury model in AC16 cells for targeted circRNAs selecting. ( A ) Cell viability of AC16 cells under different re-oxygenation times (n = 6). ( B ) LDH release of AC16 cells under different re-oxygenation times (n = 6). ( C ) Effects of H 24 h/R 1 h injury (H/RI) on cell morphology of AC16 cells (n = 3). ( D ) Effects of H/RI on ROS level in AC16 cells (n = 3). ( E ) Effects of H/RI on mRNA levels of IL-1β , IL-6 , TNF-α and ICAM-1 in AC16 cells (n = 6). ( F ) Heatmap of H/RI group compared with control group (n = 3). ( G ) The volcanic map of differentially expressed circRNAs and the candidate target circRNAs (n = 3). ( H ) Raw intensity of the candidate circRNAs in Arraystar Human CircRNA Array V2 analysis (n = 3). ( I ) RT-PCR validation results of the candidate circRNAs (n = 3). ( J ) Information of hsa_circ_0006174 (hsa_circRNA_104852) and mmu_circ_0011688. ( K ) The results of situ hybridization experiments of hsa_circ_104852 in AC16 cells and mmu_circ_ 0011688 in mouse heart tissue (n = 3). ( L ) The expression level of circMIRIAF in mouse heart tissue and mouse primary cardiomyocytes (n = 6). ..

    Expressing:

    Article Title: circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis
    Article Snippet: .. Establishment of hypoxia-reoxygenation injury model in AC16 cells for targeted circRNAs selecting. ( A ) Cell viability of AC16 cells under different re-oxygenation times (n = 6). ( B ) LDH release of AC16 cells under different re-oxygenation times (n = 6). ( C ) Effects of H 24 h/R 1 h injury (H/RI) on cell morphology of AC16 cells (n = 3). ( D ) Effects of H/RI on ROS level in AC16 cells (n = 3). ( E ) Effects of H/RI on mRNA levels of IL-1β , IL-6 , TNF-α and ICAM-1 in AC16 cells (n = 6). ( F ) Heatmap of H/RI group compared with control group (n = 3). ( G ) The volcanic map of differentially expressed circRNAs and the candidate target circRNAs (n = 3). ( H ) Raw intensity of the candidate circRNAs in Arraystar Human CircRNA Array V2 analysis (n = 3). ( I ) RT-PCR validation results of the candidate circRNAs (n = 3). ( J ) Information of hsa_circ_0006174 (hsa_circRNA_104852) and mmu_circ_0011688. ( K ) The results of situ hybridization experiments of hsa_circ_104852 in AC16 cells and mmu_circ_ 0011688 in mouse heart tissue (n = 3). ( L ) The expression level of circMIRIAF in mouse heart tissue and mouse primary cardiomyocytes (n = 6). ..

    Labeling:

    Article Title: Microglial circDlg1 modulates neuroinflammation by blocking PDE4B ubiquitination-dependent degradation associated with Alzheimer's disease
    Article Snippet: Enriched circRNAs were amplified and transcribed into fluorescent cDNA using an Arraystar Super RNA Labeling Kit (Arraystar, Rockville, MD, USA) by a random priming method. .. Then the labeled cRNAs were hybridized onto the Arraystar Mouse circRNA Array V2 (8×15K, Arraystar) and scanned by the Agilent Scanner G2505C. .. Array images were analyzed by Agilent Feature Extraction software.

    Article Title: Characterization of circRNA expression profiles and functional roles in a mouse model of liver injury induced by OSA.
    Article Snippet: .. Arraystar Mouse circRNA Array V2 was hybridized with the labeled cRNAs, which were subsequently incubated at 65 °C for 17 h. Following the washing of the slides, we scanned the array using the Agilent Scanner G2505C. .. The resulting array images were analyzed using Agilent Feature Extraction Software.

    Article Title: Expression Profiles and Bioinformatic Analysis of Circular RNAs in Db/Db Mice with Cardiac Fibrosis
    Article Snippet: The enriched circRNAs were amplified and transcribed into fluorescent complementary RNA (cRNA) using a random priming method (Arraystar Super RNA Labeling Kit, Arraystar). .. The labeled cRNAs were hybridized onto the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar). .. After the slides were washed, the arrays were scanned with the Agilent Scanner G2505C.

    Article Title: Circular RNA Profile in Atherosclerotic Disease: Regulation during ST-Elevated Myocardial Infarction
    Article Snippet: .. The enriched circRNAs were amplified and transcribed into fluorescent circRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar, Rockville, MD, USA) and hybridized onto the Arraystar Mouse circRNA Array V2 (8 × 15K, Arraystar). .. After washing, the arrays were scanned by the Agilent Scanner G2505C.

    Incubation:

    Article Title: Characterization of circRNA expression profiles and functional roles in a mouse model of liver injury induced by OSA.
    Article Snippet: .. Arraystar Mouse circRNA Array V2 was hybridized with the labeled cRNAs, which were subsequently incubated at 65 °C for 17 h. Following the washing of the slides, we scanned the array using the Agilent Scanner G2505C. .. The resulting array images were analyzed using Agilent Feature Extraction Software.

    Amplification:

    Article Title: Circular RNA Profile in Atherosclerotic Disease: Regulation during ST-Elevated Myocardial Infarction
    Article Snippet: .. The enriched circRNAs were amplified and transcribed into fluorescent circRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar, Rockville, MD, USA) and hybridized onto the Arraystar Mouse circRNA Array V2 (8 × 15K, Arraystar). .. After washing, the arrays were scanned by the Agilent Scanner G2505C.



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    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show <t>circRNA</t> expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.
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    Arraystar inc circrna microarray arraystar mouse circrna array v2
    The primers used in qRT-PCR experiments.
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    circular rna expression profiling arraystar mouse circrna array v2  (Arraystar inc)
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    a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a <t>circRNA.</t> Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h
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    Image Search Results


    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Expressing, Control, Quantitative Proteomics

    RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Quantitative RT-PCR, Microarray, Quantitative Proteomics

    circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    The primers used in qRT-PCR experiments.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: The primers used in qRT-PCR experiments.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques:

    Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques:

    QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Microarray

    DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques:

    a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a circRNA. Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h

    Journal: Cell Death & Disease

    Article Title: Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis

    doi: 10.1038/s41419-019-1590-5

    Figure Lengend Snippet: a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a circRNA. Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h

    Article Snippet: Circular RNA expression profiling was performed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, USA).

    Techniques: Expressing, Control, Amplification, Marker, Sequencing, Fluorescence, In Situ Hybridization, Staining, Quantitative RT-PCR

    The orange, purple and green nodes represent circRNA, miRNA and mRNA respectively. Markers highlighting staining showed circNRG-1-miR-193b-5p-NRG-1 interactions

    Journal: Cell Death & Disease

    Article Title: Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis

    doi: 10.1038/s41419-019-1590-5

    Figure Lengend Snippet: The orange, purple and green nodes represent circRNA, miRNA and mRNA respectively. Markers highlighting staining showed circNRG-1-miR-193b-5p-NRG-1 interactions

    Article Snippet: Circular RNA expression profiling was performed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, USA).

    Techniques: Staining